5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
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At that time, the early signs were dismissed as particles, even so the retrospective analysis shown shared characteristics with the problematic cells in society. Tracing the an infection served the lab build more laboratory processes for screening and security of assay-critical mobile strains.
As samples are acquired over the Attune CytPix Flow Cytometer, the significant-speed brightfield digicam captures and outlets photographs of detected events in a charge up to 6,000 photos for each second, according to the flow price and picture dimensions. For larger adaptability, Attune Cytometric Application helps you to regulate the image capture frequency as necessary and select a specific gate for picture seize, if necessary.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The quantity of singlets readily available for Evaluation boosts when the consumer trains and employs a far more accurate design.
Find new insights into your experimental information by visualizing populations employing combined fluorescence and morphology knowledge. Our software program interface wizards help downsampling, concatenation (merged documents) and dimensionality equipment that happen to be crafted in the software program for an intuitive, streamlined information analysis practical experience.
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Below, a highly skilled person has gated singlets confidently. Right after evaluating the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate contains more than four% aggregates.
Pair your Attune flow cytometer having an autosampler for improved workflow performance, and insert robotic integration for maximum throughput.
Consistent impression excellent even at higher throughput Acoustic concentrating along with a substantial-pace camera Mix to image these T cells continually at small or higher flow premiums. Quickly adjust concentrate and digicam options to fulfill experimental demands.
The Attune NxT cytometer is the dependable workhorse instrument your lab can rely on for reliable and trusted performance. The Attune CytPix design encompasses a large-velocity brightfield camera and AI-pushed picture analysis program, with modern upgrades together with equipment Discovering equipment and an intuitive user interface, enabling Improved data precision, precision, and more rapidly insights into sample biology.
Consumers can perspective celebration pictures in actual-time to verify sample good quality, enhance protocols, and report the morphology of each and every mobile population recognized for Examination. Imaging seize also Rewards from acoustic focusing, which really helps to position the cells making sure that a pointy, centered graphic is received.
AI-driven automatic graphic Evaluation Along with the Attune Cytometric Software package interprets occasion functions into distinct morphology parameters which might be coupled with regular fluorescence and scatter parameters to expose highly reproducible populace alterations and distinctive subsets.
We previously shown the power of imaging Car or truck-T/Ramos cell interactions. Let’s take a look at just the populace of biggest interest, the double optimistic events, to learn more. We can easily now use extended morphological parameters (here, circularity and skewness of depth) to even more analyze the characteristics of such populations and refine gating on these activities, rising facts robustness.
Leverage the strength of Superior technological know-how and innovation to accelerate your discoveries. Have faith in from the Attune Flow Cytometer spouse and children to deliver the reliability, pace, and accuracy that set your work aside.
Adaptable payment program from Agilent Economic Answers permit you to receive the important instrumentation you need for immediate venture, or to grow your lab.
At the heart of each and every Attune Flow Cytometer lies our revolutionary acoustic concentrating engineering, which makes use of seem waves to meticulously align cells inside the flow mobile. This innovative technique surpasses common hydrodynamic methods, causing quicker processing speeds, Outstanding info excellent, and elevated sample throughput.
The Attune Xenith cytometer more info is often a higher-parameter spectral analyzer, furnishing both equally spectral unmixing and conventional flow abilities for comprehensive and versatile cell Examination.
The Attune CytPix Flow Cytometer permits end users to visualize samples during acquisition, validate the precision of gating tactics, and assistance effects with responsible documentation for publication functions.
What appeared to be a homogeneous population is definitely far more assorted—an Perception that should be thought of when interpreting results.